Method of quantificationally detecting rota virus and special-purpose standard substance for the same |
Title： |
| Method of quantificationally detecting rota virus and special-purpose standard substance for the same |
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Application Number： |
200710099584 |
Application Date： |
2007/05/24 |
Announcement Date： |
2007/10/24 |
Pub. Date： |
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Publication Number： |
101058834 |
Announcement Number： |
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Grant Date： |
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Granted Pub. Date： |
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ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12Q 1/70C12Q 1/68 |
Applicant(s)： |
Tsinghua University |
Inventor(s)： |
He Miao, Hu Xiuhua, Liu Li, Shi Hanchang, Li Dan |
Key Words： |
quantificationally detecting, rota virus, standard substance |
Abstract： |
The invention discloses a quantitative detecting rotavirus method and the specific standard product. The quantitative detecting rotavirus standard product contains a nucleic acid sequence recombinant carrier of sequence 1 in the sequence table or transgenosis host cell of the recombinant carrier. The quantitative detecting rotavirus method is characterized by the following: regarding detecting sample and the genome DNA of standard product as the model or regarding detecting sample and cDNA by total RNA reverse transcription of the standard product as the model; proceeding PCR with nucleic acid sequence( sequence 2 nucleic acid fragment in the sequence table) and nucleic acid sequence( the primer comprising sequence 3 nucleic acid fragment in the sequence table); proceeding rotavirus definite quantity of the detecting sample. The standard product is provided with the association characteristic of broad spectrum identification and infectious nucleic specific, which provides good sensibility, simple manufacturing method, can be used for various sample and quick quantitative determination of rotavirus. |
Claim： |
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Priority： |
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PCT： |
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