The invention discloses a method for producing polyhydroxy fatty acid ester and a specific project bacterial, which is a recombination bacterial after diverting recombination expression carrier with cascade anaerobic induced promoter and polyhydroxy fatty acid ester synthesis gene to host bacterial. The manufacturing method of polyhydroxy fatty acid ester is: getting polyhydroxy fatty acid ester by fermentation of the project bacterial in the anoxic condition. The invention doesn't need air or oxygen in the fermentation synthesis PHA process, which also doesn't need nitrogen, reduces the cost.