Biosynthesis process of 5''-flavour nucleotide
Title:
Biosynthesis process of 5''-flavour nucleotide
Application Number:
200610165276
Application Date:
2006/12/15
Announcement Date:
2007/06/06
Pub. Date:
Publication Number:
1974780
Announcement Number:
Grant Date:
Granted Pub. Date:
ApplicationType:
Invention
State/Country:
11[China|beijing]
IPC:
C12P 19/32, C12R 1/01
Applicant(s):
Tsinghua Univ.
Inventor(s):
Xing Xinhui, Zhao Hongxi, Yang Cheng, Lu Yuan, Zhang Chong, Wang Liyan
Key Words:
Biosynthesis, 5''-flavour nucleotide
Abstract:
The present invention discloses biosynthesis process of 5'-flavour nucleotide. The thallus of Enterobacter aerogenes IAM1183 containing acid phosphatase gene is added into reaction solution containing substrate pyrophosphoric aid and carnine or guanosine to react at 25-35 deg.c and pH 4.0-8.4, so as to obtain 5'-inosinic acid or 5'-guanylic acid. The present invention has the following steps: the bacterial strain with high acid phosphatase activity, fast growth speed, powerful environment adaptation, capacity of growing in both aerobic and anaerobic condition and adaptability for industrial production; simple production process, high product yield, low cost and wide application foreground.
Claim:
Priority:
PCT:
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