This invention discloses a method for producing 1,2-epoxypropane. The method comprises: suspending mixed flora containing Methylosinus trichosporium MY9 (CGMCC No.1893) in a reaction solution, and reacting under vibration in a sealed condition with mixed gases of methane and propylene to obtain 1,2-epoxypropane. The method has such advantages as simple process, high 1, 2-epoxypropane concentration (0.85 mM/OD600), and high efficiency. The mixed flora has stable structure, rapid growth, easy culture, and high catalysis efficiency.