Horse-radish peroxidase nano-polymer biocatalyst particle and its preparation method |
Title： |
| Horse-radish peroxidase nano-polymer biocatalyst particle and its preparation method |
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Application Number： |
200610089723 |
Application Date： |
2006/07/14 |
Announcement Date： |
2007/01/31 |
Pub. Date： |
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Publication Number： |
1904042 |
Announcement Number： |
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Grant Date： |
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Granted Pub. Date： |
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ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12N 11/08, C12N 9/08 |
Applicant(s)： |
Tsinghua Univ. |
Inventor(s)： |
Yan Ming, Liu Zheng, Ge Jun, Liu Zhixia, Zhang Minlian, Ouyang Pingkai |
Key Words： |
Horse-radish peroxidase, nano-polymer biocatalyst particle, preparation method |
Abstract： |
The present invention discloses a kind of horseradish peroxidase nano high-molecular biocatalytic granules with core-shell structure, its core is horseradish peroxidase, and its external shell is a cross-linked high-molecular material, between core and shell they are connected by chemical bond. Said invention has the characteristics of high biacatalytic oxidation activity, strong thermal stability and strong resistance to organic solvent, and can be extensively used in the fields of immunoassay, organic synthesis and biological reparation, etc. Said invention also provides preparation method of above-mentioned granules. Said method includes the following steps: firstly, utilizing enzyme modification agent to introduce carbon-carbon double-bond group onto horseradish peroxidase surface, then using alkenyl monomer containing carbon-carbon double-bond as raw material, and making the above-mentioned materials undergo the process of free radical polymerization as to obtain the invented product. |
Claim： |
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Priority： |
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PCT： |
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