Abstract： |
A process for obtaining the secondary metabolite of cistanche by bioengineering technique includes such steps as preparing solid, liquid and synthetic culture media, sterilizing the cistanche tissue, inoculating it to solid culture medium, culturing its calli at 20-25 deg.C in dark condition, putting the calli in liquid culture medium, rotating culturing at 20-25 deg.C in the dark condition to obtain suspended monocells, screening high-yield monocells, inoculating them to synthetic culture medium, culturing at 20-25 deg.C in dark condition for 10-30 days, collecting cells, and separation to obtain secondary metabolite. |
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