Process for separating nucleic acid from biological particles by solid-phase carrier |
Title： |
| Process for separating nucleic acid from biological particles by solid-phase carrier |
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Application Number： |
01140445 |
Application Date： |
2001/12/07 |
Announcement Date： |
2002/06/26 |
Pub. Date： |
2007/03/14 |
Publication Number： |
1355319 |
Announcement Number： |
1304589 |
Grant Date： |
2007-3-14 |
Granted Pub. Date： |
2007-3-14 |
ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12P 19/34, C07H 21/00 |
Applicant(s)： |
Qinghua Univ |
Inventor(s)： |
Zhang Xu, Xie Xin, Chen Jiming |
Key Words： |
separating process, nucleic acid, biological particles, solid-phase carrier |
Abstract： |
A process for using solid-phase carrier to separate nucleic acid from biologic particles features that the nm-class magnetic microspheres emulsion or the specially treated fibrous membrane is used as solid-phase adsorbing medium to separate cell from biologic particle, the cell-cracking liquid is used to crack cell structure, the solid-phase adsorption and desorption are used to directly collect the biologic particles rich in nucleic acid from biologic specimen such as blood plasma, etc., and the nucleic acid is extracted. Its advantages include simple process, high speed, and easy autoamtization and miniaturization. |
Claim： |
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Priority： |
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PCT： |
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LegalStatus： |
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