Fluorescence labeling oligonucleotide probe and uses thereof |
Title： |
| Fluorescence labeling oligonucleotide probe and uses thereof |
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Application Number： |
200710122376 |
Application Date： |
2007/09/24 |
Announcement Date： |
2008/04/09 |
Pub. Date： |
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Publication Number： |
101157953 |
Announcement Number： |
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Grant Date： |
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Granted Pub. Date： |
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ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12Q 1/68 |
Applicant(s)： |
Boao Biotech Co., Ltd. |
Inventor(s)： |
Gao Huafang, Hou Wei, Wang Sixian, Cheng Jing |
Key Words： |
Fluorescence labeling, oligonucleotide probe, uses |
Abstract： |
The invention discloses a fluorescence marked obligonucleotide probe and an application of the probe. The probe of the invention changes the designing method of traditional Taqman probe that a fluorescence reporting group and a fluorescence quenching group are put at the two ends of the probe. The invention designs the fluorescence quenching group at the middle of the probe and meanwhile decorates two ends of the fluorescence quenching group with a fluorescence reporting group. Thereby, not only the distance between the fluorescence reporting group and the fluorescence quenching group is shortened, but the quenching effect is enhanced. And a molecular probe of the invention can release two molecular of fluorescence when being hydrolyzed, which can increase the strength of the fluorescence signal and enhance the detecting sensitivity. The invention can be widely applied to a real-time fluorescence PRC reaction and used for biological ship detecting. |
Claim： |
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Priority： |
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PCT： |
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