Recombined strain for producing polyhydroxy fatty ester and its construction and use |
Title: |
Recombined strain for producing polyhydroxy fatty ester and its construction and use |
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Application Number: |
03120769 |
Application Date: |
2003/03/19 |
Announcement Date: |
2004/09/29 |
Pub. Date: |
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Publication Number: |
1532278 |
Announcement Number: |
1246452 |
Grant Date: |
2006-3-22 |
Granted Pub. Date: |
2006-3-22 |
ApplicationType: |
Invention |
State/Country: |
11[China|beijing] |
IPC: |
C12N 1/21, C12N 15/63, C12N 15/11, C12N 15/31, C12P 7/62, C12R 1/01 |
Applicant(s): |
Tsinghua Univ. |
Inventor(s): |
Chen Guoqiang, Lu Xiaoyun |
Key Words: |
Recombined strain, polyhydroxy fatty ester, construction, use |
Abstract: |
The recombinant strain the present invention provides to produce 3-hydroxy butyric acid and 3-hydroxy hexylic acid copolymer with high hexylic acid ratio has introduced yafH gene of coding ester acyl CoA dehydrogenase and is microbe with capacity of synthesizing polyhydroxy aliphatic ester with aliphatic ester as substrate. The construction process of the recombinant strain includes inserting yafH gene into plasmid carrier to construct recombinant plasmid; and converting the strain with the recombinant plasmid to construct recombinant DNA strain. The present invention also provides the process of producing 3-hydroxy butyric acid and 3-hydroxy hexylic acid copolymer with high hexylic acid ratio with the recombinant strain. The present invention adopts gene recombination and microbial fermentation to produce 3-hydroxy butyric acid and 3-hydroxy hexylic acid copolymer with high hexylic acid ratio creatively and has wide practical application foreground. |
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