Method for extracting intracellular poly hydroxy fatty acid in microbe
Title：
Method for extracting intracellular poly hydroxy fatty acid in microbe
Application Number：
 200610072867
Application Date：
 2006/04/13
Announcement Date：
 2006/10/11
Pub. Date：
Publication Number：
 1844185
Announcement Number：
Grant Date：
Granted Pub. Date：
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C08G 63/06, C12P 7/64
Applicant(s)：
 Tsinghua University
Inventor(s)：
 Chen Guoqiang, Ouyang Shaoping
Key Words：
 extracting, intracellular, poly hydroxy fatty acid, microbe
Abstract：
 This invention is extraction method of cellular hydroxy fatty acid esters from microorganism. The procedure is as followings: 1) ferment to get enough microorganisms, collect it. 2) Dry to powder, add esterlike organic solvents. React at 80-120deg C for 1-4hours. Extract hydroxy fatty acid esters. 3) Restore the organic phase. 4) Precipitate hydroxy fatty acid esters from the organic phase with esterlike organic solvents. 5) Separate, wash and precipitate. 6) dry to obtain hydroxy fatty acid esters. This invention could be used in high quality PHA industrialization. It''s time-reduced, energy-saving, environment-friendly and highly-efficient. It has a large application scale.
Claim：
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  Selected patents owned by Tsinghua University filed in 2005 are loaded.
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