Method for investigating estrogen active constribution material in sewage
Title：
Method for investigating estrogen active constribution material in sewage
Application Number：
 200710062639
Application Date：
 2007/01/12
Announcement Date：
 2007/07/18
Pub. Date：
Publication Number：
 101000330
Announcement Number：
Grant Date：
Granted Pub. Date：
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 G01N 30/02G01N 33/00G01N 30/08C12Q 1/00
Applicant(s)：
 Tsinghua Univ.
Inventor(s)：
 Yu Gang, Sun Qingfeng, Deng Shubo
Key Words：
 estrogen active, constribution material, sewage
Abstract：
 The invention relates to a method used to detect estrogen activity contribution matter in sewage. It includes the following steps: extracting estrogen activity matter form the sewage sample by solid phase column extractor using methanol, dichloroethanes, normal hexane purging, and silica gel chromatographic column to classify into polarity, moderate and weak polarity three components using recombination gene yeast bioassay method to measure the estrogen activity taking out the maximum component to analyze its kind and density by gas phase color photography-mass spectrum on line. This method can fast effectively detect the main estrogen activity contribution matter in the sewage, supply foundation and support for sewerage treatment technology improvement.
Claim：
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PCT：
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2008-4-17
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