Method for producing hyaluronic acid and its special strain |
Title： |
| Method for producing hyaluronic acid and its special strain |
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Application Number： |
200410070768 |
Application Date： |
2004/07/26 |
Announcement Date： |
2005/03/02 |
Pub. Date： |
2006/07/19 |
Publication Number： |
1587387 |
Announcement Number： |
1264973 |
Grant Date： |
2006-7-19 |
Granted Pub. Date： |
2006-7-19 |
ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12N 1/21, C12N 15/74, C12N 15/87, C12R 1/46 |
Applicant(s)： |
Tsinghua Univ. |
Inventor(s)： |
Chen Guoqiang, Hao Ning |
Key Words： |
producing method, hyaluronic acid, special strain |
Abstract： |
The production process with high hyaluronic acid yield includes constructing a plasmid expressing vector containing vgb gene sequence, and introducing into streptococcus for expression. The constructed plasmid expressing vector pEUVGB is obtained through inserting the gene sequence vgb into the polyclonal site of plasmid vector pEU308. The streptococcus is preferably epizootic streptococcus. By means of expressing vgb gene in epizootic streptococcus, the present invention can raise the oxygen utilization of thallus to promote thallus growth and increase the yield of hyaluronic acid. The present invention is used in the industrial fermentation of producing hyaluronic acid, and can ensure oxygen supply and raise the yield of hyaluronic acid. |
Claim： |
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Priority： |
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PCT： |
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LegalStatus： |
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