Expression vector for siRNA of mammal cell |
Title: |
Expression vector for siRNA of mammal cell |
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Application Number: |
200310117340 |
Application Date: |
2003/12/10 |
Announcement Date: |
2005/06/15 |
Pub. Date: |
2006/08/16 |
Publication Number: |
1626663 |
Announcement Number: |
1269962 |
Grant Date: |
2006-8-16 |
Granted Pub. Date: |
2006-8-16 |
ApplicationType: |
Invention |
State/Country: |
11[China|beijing] |
IPC: |
C12N 15/85C12N 15/11C12N 5/10 |
Applicant(s): |
Tsinghua Univ. |
Inventor(s): |
Zhang Shuping, Chang Zhijie, Fu Xinyuan |
Key Words: |
Expression vector, siRNA, mammal cell |
Abstract: |
A mammal cell siRNA expression carrier existing in linear state is composed of two or three LoxP sites, the neomycin resistance gene controlled by the promoter of murine phosphoglycerate kinase gene, promoter U6 and two viscose terminals. It can be used to create the staible siRNA expression cell line of mammal and the gene silencing mouse model for silencing gene and restoring the silenced gene. |
Claim: |
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Priority: |
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PCT: |
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