Method of obtaining protein segment with independent structure/function |
Title: |
Method of obtaining protein segment with independent structure/function |
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Application Number: |
200610058019 |
Application Date: |
2006/02/24 |
Announcement Date: |
2006/08/02 |
Pub. Date: |
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Publication Number: |
1810969 |
Announcement Number: |
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Grant Date: |
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Granted Pub. Date: |
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ApplicationType: |
Invention |
State/Country: |
11[China|beijing] |
IPC: |
C12N 15/09C12N 15/63C12N 1/21C12P 21/02A61K 39/12 |
Applicant(s): |
Tsinghua Univ. |
Inventor(s): |
Lin Zhanglin, Li Shuang, Chen Yong |
Key Words: |
protein segment, independent structure/function |
Abstract: |
The method of obtaining protein segment with independent structure/function includes the following steps: 1. randomly cutting the polynucleotide sequence coding the protein and recombining to obtain random polynucleotide segments of different lengths and 2. connecting the obtained random polynucleotide segments to expression vector containing report gene, and introducing the recombinant expression vector to host for culturing to obtain the protein segment with independent structure/function. The present invention has no gene/protein specificity and simple and practical screening process, and will play important role in screening protein segment with independent structure/function and preparing virus antigen and virus vaccine. |
Claim: |
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Priority: |
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PCT: |
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