Recombinant bacterium with granular methane monooxygenase activity and application thereof |
Title： |
| Recombinant bacterium with granular methane monooxygenase activity and application thereof |
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Application Number： |
200510098409 |
Application Date： |
2005/09/06 |
Announcement Date： |
2006/04/26 |
Pub. Date： |
2007/08/01 |
Publication Number： |
1763178 |
Announcement Number： |
1329506 |
Grant Date： |
2007-8-1 |
Granted Pub. Date： |
2007-8-1 |
ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12N 1/21, C12N 15/52, C12N 15/62, C12N 15/74, C12N 9/00, C12P 7/02 |
Applicant(s)： |
Tsinghua University |
Inventor(s)： |
Gou Zhongxuan, Luo Mingfang, Xing Xinhui |
Key Words： |
granular, methane monooxygenase activity, recombinant bacterium, application |
Abstract： |
The present invention discloses one kind of recombinant bacterium with granular methane monooxygenase activity and its application. Fusion gene comprising the promoter sequences of coding gene and desulfurizing gene of granular methane monooxygenase is introduced into Rhodococcus, and after screening, the strain expressing granular methane monooxygenase or the recombinant bacterium with granular methane monooxygenase activity is obtained. The promoter sequence of the desulfurizing gene is located in the upstream of the granular methane monooxygenase coding gene. The recombinant bacterium avoids the effect of methanol dehydrogenase radically, makes methane oxidation stop in methanol reaction step, and is superior to wild methane oxidizing bacteria and easy to culture. The recombinant bacterium can convert alkane in natural gas into liquid alcohol, convert methane into methanol and oxidize propylene into 1, 2-epoxy propane. |
Claim： |
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Priority： |
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PCT： |
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LegalStatus： |
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