Method of expressing heparinase and its special expression carrier |
Title： |
| Method of expressing heparinase and its special expression carrier |
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Application Number： |
200510090872 |
Application Date： |
2005/08/18 |
Announcement Date： |
2006/04/12 |
Pub. Date： |
2007/12/19 |
Publication Number： |
1757739 |
Announcement Number： |
100355893 |
Grant Date： |
2007-12-19 |
Granted Pub. Date： |
2007-12-19 |
ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12N 15/63, C12N 15/65, C12N 15/62, C12N 15/70, C12N 1/21, C12N 9/24, C07K 19/00 |
Applicant(s)： |
Tsinghua Univ. |
Inventor(s)： |
Xing Xinhui, Chen Yin, Kuang Ying, Ye Fengchun, Luo Mingfang |
Key Words： |
heparinase, expressing method, special, expression carrier |
Abstract： |
A method for expressing heparinase and its dedicated expression carrier are disclosed. Said heparinase expression carrier is a proerythroblast expression carrier for the coding gene of the fusion protein composed of heparinase and green fluorescin, and can be used for soluble expression and be correctly folded to prevent the generation of inclusion body. It can also be used to fast and quantitatively test the bacterial density and heparinase activity. Said method can realize the efficient soluble expression of said fusion protein (GFP-hepA). |
Claim： |
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Priority： |
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PCT： |
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LegalStatus： |
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