Asymmetrical PCR amplification method, dedicated primer and use thereof
Title：
Asymmetrical PCR amplification method, dedicated primer and use thereof
Application Number：
 200410056866
Application Date：
 2004/08/26
Announcement Date：
 2005/06/22
Pub. Date：
 2006/10/18
Publication Number：
 1629305
Announcement Number：
 1280422
Grant Date：
 2006-10-18
Granted Pub. Date：
 2006-10-18
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C12P 19/34, C12Q 1/68
Applicant(s)：
 CapitalBio Corporation
Inventor(s)：
 Zhang Zhiwei, Wang Can, Zhu Lingxiang
Key Words：
 Asymmetrical PCR, amplification method, dedicated primer, use
Abstract：
 The invention discloses an asymmetrical PCR amplification method, dedicated primer and use thereof, wherein the primer 5' end of the primer pairs is added by a segment of oligonucleotide tail in irrelevancy with the sequence irrelevant sequence irrelevancy to be expanded, the asymmetrical PCR expansion process comprises the steps of, (1) predenaturizing, (2) PCR expanding for first part containing denaturized, primer annealing and primer expansion, (3) a second part PCR expansion of containing a plurality of degenerated and primer extension cycle. The invention realizes high yield and multiple PCR expansion, thus can be used widely in nucleic acid detection.
Claim：
Priority：
PCT：
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