Coli somatic polyclonal antibody, and its preparing method and use
Title：
Coli somatic polyclonal antibody, and its preparing method and use
Application Number：
 200610113827
Application Date：
 2006/10/18
Announcement Date：
 2007/03/28
Pub. Date：
Publication Number：
 1935843
Announcement Number：
Grant Date：
Granted Pub. Date：
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C07K 16/12, G01N 33/535
Applicant(s)：
 Tsinghua Univ.
Inventor(s)：
Key Words：
 Coli somatic, polyclonal antibody, preparing method and use
Abstract：
 The invention discloses Escherichia coli thalli polyclonal antibody and its preparation method and application. The preparation method includes the following steps: separating out the Escherichia coli; putting it into physiological saline with asepsis glass bead and/or steel ball, waving, hatching at 80-120 degree centigrade for 1.5-3h, centrifuge, upper heat removing, sterilizing after PBS to gain Escherichia coli antigen; using it to immune animal; separating and purify the antiserum of the animal to gain the Escherichia coli thalli polyclonal antibody which has the advantages of high specificity, purity, titer( over 6400), saving for long, high precision, sensibility and less detecting steps for Escherichia coli by immunoassay in environment and food detecting field, wide application prospect.
Claim：
Priority：
PCT：
LegalStatus：

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  Selected patents owned by Tsinghua University filed in 2005 are loaded.
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