This invention discloses a method for preparing polyacrylamide macroporous gel carrier for immobilized cells. The method comprises: preparing a mixed solution of acrylamide monomer and N,N'-methylene bis-acrylamide monomer, adding NaCl into the mixed solution to saturation, transferring the mixed solution into a container for preparing gel carrier, uniformly adding NaCl powder to 2-5 cm above the solution surface, exhausting oxygen in the mixed solution and in the container, sealing the container, radiating with radioactive rays so that acrylamide monomer and N,N'-methylene bis-acrylamide monomer polymerize to obtain a gel, and heating the gel in a water bath to obtain polyacrylamide macroporous gel carrier for immobilized cells. The polyacrylamide macroporous gel carrier can be used in immobilization of microbes, such as a mixture of bacteria and fungi, and mixed bacteria for cellulose degradation.