Escherichia coli self-cracking method and its dedicated carrier and application
Title：
Escherichia coli self-cracking method and its dedicated carrier and application
Application Number：
 200610078951
Application Date：
 2006/04/28
Announcement Date：
 2006/12/20
Pub. Date：
Publication Number：
 1880460
Announcement Number：
Grant Date：
Granted Pub. Date：
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C12N 15/70, C12N 15/31, C12N 15/33, C12N 1/21, C12N 9/00
Applicant(s)：
 Tsinghua University
Inventor(s)：
 Lin Zhanglin, Xu Lihua, Li Shuang, Ren Chuan
Key Words：
 Escherichia coli, self-cracking, dedicated carrier, application
Abstract：
 The invention discloses a colibacillus self-splitting method and specific carrier and appliance, which is characterized by the following: the carrier connects colibacillus expressing carrier with heat promoter, bacteriophage splitting gene and colibacillus terminal from 5' to 3'sequently; the colibacillus self-splitting carrier is conducted in the colibacillus to obtain recombinant colibacillus, which is shined by heat to split colibacillus cell. The experiment certifies that the splitting gene SRRz of Lambda bacteriophage can be expressed under fitful temperature induced condition.
Claim：
Priority：
PCT：
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