Engineering bacteria for expressing benzene ring bioxygerase and its application |
Title： |
| Engineering bacteria for expressing benzene ring bioxygerase and its application |
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Application Number： |
200610080606 |
Application Date： |
2006/05/19 |
Announcement Date： |
2006/11/29 |
Pub. Date： |
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Publication Number： |
1869200 |
Announcement Number： |
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Grant Date： |
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Granted Pub. Date： |
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ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12N 1/21, C12N 15/53, C12P 7/02, C12P 1/04, C12R 1/38 |
Applicant(s)： |
Tsinghua Univ. |
Inventor(s)： |
Chen Guoqiang, Ouyang Shaoping, Sun Shangyu, Chen Jinchun |
Key Words： |
Engineering bacteria, expressing, benzene ring bioxygerase, application |
Abstract： |
The invention discloses an engineering fungus, which could express benzene ring dioxygenase, and the application. Especially, it involves the special expressing engineering fungus of benzene ring dioxygenase and the application in producing syn-1, 2-dihybric-3, 5-cyclohexane. The engineering fungus is gained by inducing the expressing carrier containing benzene ring dioxygenase gene into fake single cell fungus. The producing method of syn-3, 5-dihyadric-1, 2-cyclohexadiene contains the following steps: fermenting the engineering fungus to gain benzene ring dioxygenase adding substrate benzene or benzene solution, and the content adding to fermenting liquid is 10-30mL/L every hour fermenting for 8-24 hours to gain syn-1, 2-dihybric-3, 5-cyclohexane solvent of the benzene solution could be olefin or bean oil, and the volume ratio of benzene and solvent is 1:1-3. The special expressing engineering fungus of benzene ring dioxygenase will perform an important function in the industrial producing of DHCD, and it has great application prospect. |
Claim： |
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PCT： |
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