Method of domesticating and screening benzene series anaerobic degradation flora
Title：
Method of domesticating and screening benzene series anaerobic degradation flora
Application Number：
 200710063095
Application Date：
 2007/01/26
Announcement Date：
 2007/08/15
Pub. Date：
Publication Number：
 101016532
Announcement Number：
Grant Date：
Granted Pub. Date：
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C12N 1/20C02F 3/28C02F 11/04C09C 1/10
Applicant(s)：
 Tsinghua University
Inventor(s)：
 Liu Xiang, Dou Junfeng, Deng Dong
Key Words：
 benzene series, anaerobic degradation, flora, domesticating, screening
Abstract：
 The invention discloses a domestication screening method and appliance of benzene series anaerobic degraded flora in polluted soil and water biological degraded disposing technical domain, which comprises the following steps: adding electron acceptor, sodium sulfide and BTEX mixture into polluted soil sealing and stewing at three months adding basic culture medium, trace metal liquid and vitamin C solution into anaerobic box filled with nitrogen domesticating soil switching domestication soil into anaerobic bottle with mass ratio of water and soil in the anaerobic box adding into BTEX, electron acceptor and sodium sulfide with mass ratio culturing 7-9 days in rotating oscillator with the temperature at 20+-2 deg.c circling at least 9 times getting the degraded flora. This invention can screen good anaerobic degraded property microbe flora, which can make degraded speed range of BTEX at 1.6-3.5mg.L-1.d-1.
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