Connection type microbial cultivation device with embedded millipore filter and culturing method thereof
Title：
Connection type microbial cultivation device with embedded millipore filter and culturing method thereof
Application Number：
 200710099489
Application Date：
 2007/05/23
Announcement Date：
 2008/01/23
Pub. Date：
Publication Number：
 101109006
Announcement Number：
Grant Date：
Granted Pub. Date：
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C12M 3/06
Applicant(s)：
 Tsinghua University
Inventor(s)：
 Wu Xiaolei, Guo Bin, Yu Sulin
Key Words：
 cultivation device, culturing method, millipore, filter, microbial
Abstract：
 The invention discloses a built-in microporous filter membrane connection microbe culture device belonging to the microbe culture technology, a culture method thereof, and a microbe culture, isolation and purification and observation technology in various environments by utilizing a culture medium. The culture device is provided with clapboard in an internal space composed of a dish body and a dish body lid, and the clapboard is fixed with a microporous filter membrane which divides the internal space of the culture device into two spaces. The two spaces are respectively used to bear the microbial culture group and provide a simulation external environment medium of a microchemistry matter with a microbial growth necessary factor. The dissolved matters at the two sides of the filter membrane are capable of freely exchange and the cell cannot permeate. Therefore, the culture device is capable of culturing more miceobes that the regular method can not culture. The invention applies the culture device to culture the microbes, which can effectively improve the culturablity of the environmental microbes, facilitate experiment system construction, make the operation easy and time-saving and has stronger industrialized application potention.
Claim：
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PCT：
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