T carrier and its construction method
Title:
T carrier and its construction method
Application Number:
200410047905
Application Date:
2004/06/10
Announcement Date:
2005/03/09
Pub. Date:
Publication Number:
1590550
Announcement Number:
Grant Date:
Granted Pub. Date:
ApplicationType:
Invention
State/Country:
11[China|beijing]
IPC:
C12N 15/63, C12N 15/64, C12N 15/66
Applicant(s):
Tsinghua Univ.
Inventor(s):
Chen Qijun, Wang Xuechen, Zhou Haimeng
Key Words:
T carrier, construction method
Abstract:
A T carrier with higher TA cloning efficiency includes the Amp gene sequence of pBlue Script II SK(-), the ColEI ori sequence of pBlue Script II SK (-), the flori sequence of pBlue Script II SK(-), the lacZ gene sequence of pBlue Script IISK (-), the T3 bacteriophage promoter sequence of pBlue Script II SK (-), T7 bacteriophage promoter sequence of pBlue Script II SK(-), and two polycloning site sequences at both ends. The process for preparing T carrier is also disclosed.
Claim:
Priority:
PCT:
LegalStatus:

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2008-4-17
  Selected patents owned by Tsinghua University filed in 2005 are loaded.
2008-3-31
  Selected patents owned by Tsinghua University filed in 2006 and 2007 are load.







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