T carrier and its construction method |
Title: |
T carrier and its construction method |
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Application Number: |
200410047905 |
Application Date: |
2004/06/10 |
Announcement Date: |
2005/03/09 |
Pub. Date: |
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Publication Number: |
1590550 |
Announcement Number: |
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Grant Date: |
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Granted Pub. Date: |
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ApplicationType: |
Invention |
State/Country: |
11[China|beijing] |
IPC: |
C12N 15/63, C12N 15/64, C12N 15/66 |
Applicant(s): |
Tsinghua Univ. |
Inventor(s): |
Chen Qijun, Wang Xuechen, Zhou Haimeng |
Key Words: |
T carrier, construction method |
Abstract: |
A T carrier with higher TA cloning efficiency includes the Amp gene sequence of pBlue Script II SK(-), the ColEI ori sequence of pBlue Script II SK (-), the flori sequence of pBlue Script II SK(-), the lacZ gene sequence of pBlue Script IISK (-), the T3 bacteriophage promoter sequence of pBlue Script II SK (-), T7 bacteriophage promoter sequence of pBlue Script II SK(-), and two polycloning site sequences at both ends. The process for preparing T carrier is also disclosed. |
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Priority: |
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PCT: |
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Relevancy information |
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Other Patents of Same Inventor |
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Last Update |
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2008-4-17 |
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Selected patents owned by Tsinghua University filed in 2005 are loaded. |
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2008-3-31 |
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Selected patents owned by Tsinghua University filed in 2006 and 2007 are load. |
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