Method for producing 7-amino cephalosporanic acid and its specific recombinase |
Title： |
| Method for producing 7-amino cephalosporanic acid and its specific recombinase |
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Application Number： |
200310113563 |
Application Date： |
2003/11/17 |
Announcement Date： |
2004/11/10 |
Pub. Date： |
2006/05/24 |
Publication Number： |
1544620 |
Announcement Number： |
1257269 |
Grant Date： |
2006-5-24 |
Granted Pub. Date： |
2006-5-24 |
ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12N 9/00C12N 15/52C12P 35/00 |
Applicant(s)： |
Tsinghua University |
Inventor(s)： |
Luo hui, Li Qiang, Yu Huimin |
Key Words： |
7-amino cephalosporanic acid, producing method, recombinase |
Abstract： |
The invention discloses a method of producing 7-amido-cephalosporanic acid and its special recombinant enzyme, providing a recombinant enzyme with the activity of glutaryl-7-amido- cephalosporanic acid acylase and D-amino acid oxidase and its coding gene, and a method of using the recombinant enzyme to catalyze cephalosporin C so as to directly produce 7-amido- cephalosporanic acid. The recombinant enzyme is a protein with aminophenol residual group order of SEQID No:6 in the order table a protein obtained by making an or several aminophenol residual group substitutions, deletion or addition on the aminophenol residual group order of SEQID No:6, derived from SEQID No:6 and having the same activity as the aminophenol residual group order of SEQID No:6. The method of producing 7-amido-cephalosporanic acid uses the recombinant enzyme to catalyze cephalosporin C so as to produce 7-amido- cephalosporanic acid. The recombinant enzyme can be widely applied to production of 7-amido- cephalosporanic acid. It provides a new way for the recombinant enzyme catalyzing cephalosporin C to produce 7-amido- cephalosporanic acid. |
Claim： |
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Priority： |
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PCT： |
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LegalStatus： |
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