1,3-propylene glycol and 2,3-butanediol production method using glecerol by microorganism two-step fermentation method |
Title： |
| 1,3-propylene glycol and 2,3-butanediol production method using glecerol by microorganism two-step fermentation method |
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Application Number： |
200410037692 |
Application Date： |
2004/04/29 |
Announcement Date： |
2005/01/26 |
Pub. Date： |
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Publication Number： |
1570123 |
Announcement Number： |
1246465 |
Grant Date： |
2006-3-22 |
Granted Pub. Date： |
2006-3-22 |
ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12P 7/18 |
Applicant(s)： |
Tsinghua University |
Inventor(s)： |
Liu Dehua, Sun Yan, Cheng Keke |
Key Words： |
1,3-propylene glycol, 2,3-butanediol, glecerol, microorganism, two-step fermentation method |
Abstract： |
The invention discloses a method for simultaneously preparing 1,3-propylene glycol and 2,3-butylene glycol from glycerol by using microbe employing prior-stage anaerobic and later-stage aerobic two-stage fermentation process, which belongs to the field of the bio-chemical engineering technology. In the fermentation process, the seed liquid is added in the primary fermentation medium containing industrial glycerol or glycerol fermentation liquid, after 3¡«5 hours, the mixed liquid of glycerol and glucose is added in the form of the flow. The product concentrations of 1,3-propylene glycol and 2,3-butylene glycol are controlled by adjusting the air flow in the two-stage fermentation process and controlling the fermentation temperature. When the fermentation process is over, 1,3-propylene glycol and 2,3-butylene glycol are separated and purified by desalting, distilling, and vacuum rectification. The preparing method can improve the fermentation concentration and the yield of 1,3-propylene glycol and the production intensity, at the same time, 2,3-butylene glycol with higher concentration can be obtained with high raw material availability and low cost. |
Claim： |
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Priority： |
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PCT： |
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