Expression carrier of low oxygen inducing microorganusm and constructure process and application thereof |
Title： |
| Expression carrier of low oxygen inducing microorganusm and constructure process and application thereof |
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Application Number： |
200410006124 |
Application Date： |
2004/03/03 |
Announcement Date： |
2005/01/05 |
Pub. Date： |
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Publication Number： |
1560256 |
Announcement Number： |
1246463 |
Grant Date： |
2006-3-22 |
Granted Pub. Date： |
2006-3-22 |
ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12N 15/63, C12N 15/70, C12N 15/79 |
Applicant(s)： |
Tsinghua Univ. |
Inventor(s)： |
Chen Guoqiang, Liu Tao, Chen Jingyu |
Key Words： |
Expression carrier, low oxygen inducing, microorganusm, constructure process, application |
Abstract： |
The invention discloses a hypoxia-induced microbe expression carrier and its constructing mehtd and application, and it is a microbe particle expression carrier containing a vgb starter sequence or a nar starter sequence in E. coli regulated and controlled by fumarate and nitrate reductase mediator FNR. The constructing method: connecting the vgb or nar starter sequence at a polyclonal site of microbe expression carrier to obtain it. As using the expression carrier for fermenting, it can achieve the effect of still keeping stable plasmid inheritance without adding in antibiotics; it can meet the demand of cell growth on high-dissolved oxygen, reducing the requirement of stirring and aerating; it can conveniently and economically induce the expression of exogenous object protein, having a wide prospect in industrial application. |
Claim： |
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Priority： |
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PCT： |
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LegalStatus： |
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