Engineering bacterium for expressing poly hydroxy fatty acid ester and its construction method and application |
Title: |
Engineering bacterium for expressing poly hydroxy fatty acid ester and its construction method and application |
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Application Number: |
200710100153 |
Application Date: |
2007/06/05 |
Announcement Date: |
2008/01/02 |
Pub. Date: |
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Publication Number: |
101096651 |
Announcement Number: |
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Grant Date: |
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Granted Pub. Date: |
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ApplicationType: |
Invention |
State/Country: |
11[China|beijing] |
IPC: |
C12N 1/21C12P 7/62 |
Applicant(s): |
C12N 1/21C12P 7/62 |
Inventor(s): |
Chen Guoqiang, Ouyang Shaoping, Chen Sisi, Liu Qian |
Key Words: |
Engineering bacterium, poly hydroxy fatty acid ester, construction method, application |
Abstract: |
The invention discloses a method for expressing polyhydroxy fatty acid ester and a specific project bacterial, wherein the project bacterial for expressing polyhydroxy fatty acid ester is the mutant of the polyhydroxy fatty acid ester bacterial by inhibiting or removing a or a plurality genes related to fatty acid beta oxidization metabolic pathway in the polyhydroxy fatty acid ester bacterial. The method comprises the following steps: fermenting the project bacterial for expressing polyhydroxy fatty acid ester with the fermentation culture medium of 5-20g/l 5-18 carbon chain length fatty acid. The method has the simple construction method, which has the simple operation, the high yield, the low cost and the short production cycle. The project material has the wide application prospect. |
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