Method for producing saussurea involucrate secondary metabolites
Title：
Method for producing saussurea involucrate secondary metabolites
Application Number：
 200710121086
Application Date：
 2007/08/29
Announcement Date：
 2008/03/12
Pub. Date：
Publication Number：
 101139571
Announcement Number：
Grant Date：
Granted Pub. Date：
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C12N 5/04
Applicant(s)：
 Tsinghua University
Inventor(s)：
 Guo Zhigang, Zhao Lin
Key Words：
 producing method, saussurea involucrate, secondary metabolites
Abstract：
 The invention discloses a method for producing secondary metabolities of saussurea involucrata, which comprises such procedures as: 1) culturing in a dedifferentiation way the outer colonizing body of saussurea involucrata to get wound-recovering tissue of saussurea involucrata, taming the wound-recovering tissue of saussurea involucrata to get a loose and rapidly-growing cell system; 2) inducing and culturing in a basic culture media with addition of colchicin the cell system of wound-recovering tissue of saussurea involucrata got in 1) to get a polyploid cell system, then culturing in an expansive way to get a lot of polyploid cells of saussurea involucrata; 3) inoculating the polyploid cells of saussurea involucrate got from 2) in a synthesizing culture media to get cells rich with secondary metabolities of saussurea involucrate. The method in the invention is for culturing and producing secondary metabolities of saussurea involucrata by using saussurea involucrata cells, can steadily achieve the industrialized production of such metabolities of saussurea involucrata as saussurea involucrata flavone. The invention has a low production cost, short culture period, is not influenced by natural environment and climate, and can achieve yearly production.
Claim：
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PCT：
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