Method for gaining panax japonicus secondary metabolite by using panax japonicus cell culture and biological transformation technique
Title：
Method for gaining panax japonicus secondary metabolite by using panax japonicus cell culture and biological transformation technique
Application Number：
 200710117741
Application Date：
 2007/06/22
Announcement Date：
 2008/01/30
Pub. Date：
Publication Number：
 101113430
Announcement Number：
Grant Date：
Granted Pub. Date：
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C12N 5/04
Applicant(s)：
 Tsinghua University
Inventor(s)：
 Guo Zhigang, Sun Ruiqiang
Key Words：
 panax japonicus, secondary metabolite, cell culture, biological transformation
Abstract：
 The invention discloses a method by using panax japonicus cell culture and biological transfer technology to obtain secondary metabolites and pertains to plant cell culture engineering field. An improved RW culture medium is added with crane sugar, plant growth hormone and cytokinin and then coagulant; the obtained mixture is made into an induction culture medium after being disinfected in high temperature and then cooled; explants of panax japonicus are inoculated in the induction culture medium under an aseptic condition to be inducted to form callus; after an expansion, a plurality of cells are obtained; the expanded callus in the synthetic medium is inoculated into synthetic culture solution and then panax japonicus saponin crude extracts and byproducts thereof can be obtained through the extraction of cells from the solvent, thereafter the panax japonicus saponin, panax japonicus polysaccharide and other compounds can be obtained through purification. By adopting the method of the invention, secondary metabolites of the panax japonicus can be produced with low cost, short culture cycle, year-round production and no natural environmental limit.
Claim：
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PCT：
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