Combination gene probe for detecting iron oxidizing bacteria and detecting method thereof |
Title： |
| Combination gene probe for detecting iron oxidizing bacteria and detecting method thereof |
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Application Number： |
200410097123 |
Application Date： |
2004/12/10 |
Announcement Date： |
2005/07/06 |
Pub. Date： |
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Publication Number： |
1635166 |
Announcement Number： |
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Grant Date： |
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Granted Pub. Date： |
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ApplicationType： |
Invention |
State/Country： |
11[China|beijing] |
IPC： |
C12Q 1/68 |
Applicant(s)： |
Tsinghua University |
Inventor(s)： |
Wu Xiaolei, Qu Rui, Yan Yun |
Key Words： |
Combination gene probe, iron oxidizing bacteria, detecting method |
Abstract： |
The invention relates to a combined gene probe for detecting iron bacteria (IB) and detection method thereof, belongs to microorganism detection technology field. The combined gene probe is composed by at least two gene orders of the 4 ones in IBs; the detection method comprises: sampling water, fixing the microbial cells in water sample by iso-polyaldehyde solution, instilling it into slide, drying, dewatering, adding the combined gene probe solution, adding mixed solution of NaCl and formamide; hybridizing, washing the probe solution on the slide by phosphate buffer solution, washing the water sample on the slide by distilled water, dying, observing the biological sample on the slide by fluorescence microscope, and counting the IBs cells with fluorescence. The invention can detect the IBs in re-injected water from oil field rapidly, allsidedly, accurately and conveniently, and can realize the timely and accurate control for oil recovery process. |
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| Last Update |
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2008-4-17 |
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Selected patents owned by Tsinghua University filed in 2005 are loaded. |
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2008-3-31 |
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Selected patents owned by Tsinghua University filed in 2006 and 2007 are load. |
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