Combination gene probe for sulphate reduction pronucleus bioinstrumentation and detecting process thereof
Title：
Combination gene probe for sulphate reduction pronucleus bioinstrumentation and detecting process thereof
Application Number：
 200410086710
Application Date：
 2004/10/29
Announcement Date：
 2005/07/06
Pub. Date：
 2006/08/23
Publication Number：
 1635162
Announcement Number：
 1271217
Grant Date：
 2006-8-23
Granted Pub. Date：
 2006-8-23
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 C12Q 1/68
Applicant(s)：
 Tsinghua University
Inventor(s)：
 Wu Xiaolei, Zeng Jinghai, Qu Rui
Key Words：
 Combination gene probe, sulphate, reduction, pronucleus bioinstrumentation, detecting process
Abstract：
 The invention relates to a combined gene probe for sulfate reduction pronucleus biological detection and the detecting method thereof, belongs to microorganism detection technology field. The combined gene probe is composed by at least two gene orders of the 8 ones in SRPs; the detection method comprises: sampling water, fixing the microbial cells in water sample by iso-polyaldehyde solution, instilling it into slide, drying, dewatering, adding the combined gene probe solution, adding hybridization solution of NaCl and formamide, hybriding, washing the probe solution on the slide by phosphate buffer solution, washing the water sample on the slide by distilled water, dying, observing the biological sample on the slide by fluorescence microscope, and counting the SRPs cells with fluorescence. The invention can detect the SRPs in discharged water from oil field rapidly, allsidedly, accurately and conveniently, and can realize the timely and accurate control for oil recovery process.
Claim：
Priority：
PCT：
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