Human tyrosinase expression carrier and its use
Title:
Human tyrosinase expression carrier and its use
Application Number:
200410088717
Application Date:
2004/11/01
Announcement Date:
2005/04/06
Pub. Date:
2006/10/18
Publication Number:
1603417
Announcement Number:
1280417
Grant Date:
2006-10-18
Granted Pub. Date:
2006-10-18
ApplicationType:
Invention
State/Country:
11[China|beijing]
IPC:
C12N 15/63, C12R 1/84, C12N 15/81, C12N 9/00, C12N 15/52, C12N 1/19
Applicant(s):
Tsinghua University
Inventor(s):
Chen Guoqiang, Wang Hongtao, Li Zhenguo
Key Words:
Human tyrosinase, expression carrier, use
Abstract:
The invention discloses a kind of tyrosinase to express the carrier and its the application, its goal was provides a kind of person tyrosinase to express the carrier and to induct this carrier the reorganization pasteur to finish the red yeast cell and to use this reorganization cell to produce the person tyrosinase the method. The above-mentioned person tyrosinase expresses the carrier is includes the person tyrosinase gene and the Histag gene pasteur finishes the red yeast to express the carrier. Expresses the person tyrosinase the method, pasteur finishes the red yeast cell including the reorganization, the methyl alcohol induction expresses the person tyrosinase and kisses using the metal with the chromatographic analysis separation purifies person tyrosinase the step. The invention person tyrosinase gene expresses the carrier to cause the person tyrosinase gene conformity through the homologous reorganization to enter pasteur finishes the red yeast cell in the gene group, stably expresses the person tyrosinase, moreover does not need in the fermentative process to use the expensive antibiotic and IPTG, causes the production cost greatly to reduce, in addition, pasteur finishes the red yeast the high density growth, also is advantageous to the person tyrosinase expression, the expression quantity may reach 0.39g/L.
Claim:
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PCT:
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