Multiple wavelength Chinese traditional medicine chromatogram fingerprint, fabricating method and application thereof
Title：
Multiple wavelength Chinese traditional medicine chromatogram fingerprint, fabricating method and application thereof
Application Number：
 200310103862
Application Date：
 2003/11/14
Announcement Date：
 2004/11/10
Pub. Date：
 2006/10/25
Publication Number：
 1544935
Announcement Number：
 1281948
Grant Date：
 2006-10-25
Granted Pub. Date：
 2006-10-25
ApplicationType：
 Invention
State/Country：
 11[China|beijing]
IPC：
 G01N 30/02G01N 30/72G01N 30/74G01N 30/86G01N 21/31G01N 33/15
Applicant(s)：
 Tsinghua University
Inventor(s)：
 Luo Guoan, Yang Xuedong, Cao Jin
Key Words：
 Chinese traditional medicine, chromatogram, fingerprint, fabricating method, application
Abstract：
 The invention relates to a multi-wavelength Chinese medicinal chromatographic fingerprint pattern and its constructing method and application, by investigation and optimization of chromatographic conditions and UV response, determining the gradient curve and many UV detected wavelengths within the eluting time of 1-1.5 hours to measure the fingerprint pattern and make chemical verification; adopting relative response valve and quantifiable peak as fixed-point indexes to make relativized processing and matrixing, so as to establish quantized multi-wavelength fingerprint pattern shared-mode data matrix; making similarity identification and component difference comparison on the unknown sample by the matrix, so as to implement quality identification and evaluation, qualitatively and quantitatively. Its advantages: it can not only grasp varieties and quality conditions of Chinese medicines by the whole chromatographic characteristic, but also better characterize the peculiarity of Chinese medicinal components by multi-wavelength selection and fingerprint peak quantitative identification, and strengthens the expression ability and standard degree of the fingerprint pattern for varieties and quality of Chinese medicines by quantizing main components. The established fingerprint pattern can be widely applied to Chinese medicinal quality detection and evaluating technique fields.
Claim：
Priority：
PCT：
LegalStatus：
 2008-1-9Date of termination due to fee

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