Non-immetsed type liquid fermentation process of lingin catabolic enzymes from Phanerochaete chrysosporium |
Title: |
Non-immetsed type liquid fermentation process of lingin catabolic enzymes from Phanerochaete chrysosporium |
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Application Number: |
200410003496 |
Application Date: |
2004/04/02 |
Announcement Date: |
2005/01/12 |
Pub. Date: |
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Publication Number: |
1563369 |
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Grant Date: |
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Granted Pub. Date: |
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ApplicationType: |
Invention |
State/Country: |
11[China|beijing] |
IPC: |
C12N 9/24 |
Applicant(s): |
Tsinghua Univ. |
Inventor(s): |
Wen Xianghua, Yu Guoce, Xiong Xiaoping |
Key Words: |
Non-immetsed type, liquid fermentation, lingin catabolic enzymes, Phanerochaete chrysosporium |
Abstract: |
This invention relates to thelephoroid lignin non-immersion liquid fermentation method which inlets the thelephoroid liquid seeds in a liquid culture medium with carrier to be cultivated in oscillation under air atmosphere of 35-39deg.C. Said solid cultivation carrier is either inert or non-inert and a 0.5-2.5cm size block. Stacked height of the carrier is higher than the surface of the liquid culture medium for about 1-5times higher and the carrier is at un-immersion state. Said fermentation culture medium adopts either carbon limit liquid medium or nitrogen limit liquid culture medium PH is controlled at 4.0-6.0 the medium contains reratryl alcohol of 0-2.5mM. |
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